Histamine H1 assay (GDH12)
| Type of assay | Cellular |
| Receptor: | Human histamine H1 |
| Expression system: | Transient transfection of HEK-293 |
| Reporter gene | NFAT-firefly luciferase |
| Experiment settings: | - 8 concentrations of test compounds, each in triplicate – total reaction volume of 200 µL – total reaction volume of 200 µL – 1 µM histamine (only for antagonism) – incubation at 37°C for 6 hours |
| Read-out: | Bioluminescence |
| References: | Histamine (EC50 = 121 nM) Mepyramine (IC50 = 20 nM at 1 µM histamine) |
| Z’ factor | >0.7 |
