Histamine H2 assay (GDH21)
| Type of assay | Biochemical |
| Receptor: | Human Histamine H2 |
| Expression system: | Transient transfection of HEK-293T |
| Radioligand: | [3H]tiotidine |
| Radioligand concentration: | 2 nM |
| Kd (nM): | 12 nM |
| Experiment settings: | - 8 concentrations of test compounds, each in triplicate – total reaction volume of 200 µL – incubation at RT for 1 hour |
| Read-out: | Scintillation counting, converted in dpm unit |
| Reference: | Ranitidine (Ki = 117 nM), tiotidine (Ki = 25 nM) |
| Z’ factor | >0.7 |
