Histamine H4 assay (GDH43)
| Type of assay | Biochemical |
| Receptor: | Mouse histamine H4 receptor |
| Expression system: | Transient transfection of HEK-293T |
| Radioligand: | [3H]histamine |
| Radioligand concentration: | 25 nM |
| Kd (nM): | 78 nM |
| Experiment settings: | - 8 concentrations of test compounds, each in triplicate – total reaction volume of 200 µL – incubation at RT for 1 hour |
| Read-out: | Scintillation counting, converted in dpm unit |
| Reference: | JNJ 7777120 (pKi = 8.4) |
| Z’ factor | >0.5 |
